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Peptides were dissolved in running buffer and the stock concentrations determined using the NanoDrop 2000 spectrophotometer (Thermo Fischer Scientific) at a wavelength of 280 nm

The total coat state score was calculated by summing the individual scores from all body regions, with higher scores indicating poorer coat condition

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During weeks 12-24, lighter periods, improved skin clarity, easier weight management, and fewer hormonal symptoms develop

GDNF preferentially binds to GDNF family receptor (GFR) to form a complex, which then associates with the Ret receptor, triggering downstream signaling cascades such as PI3K/Akt, MAPK/ERK, and Src kinase pathways, thereby exerting trophic effects on dopaminergic neurons [13] (Fig